Anaerobic bacteria belong to the main normal flora in the human body, and are more common in the human oral cavity, intestines, tumors, and urinary tract; Fusobacterium mainly exists in the upper respiratory tract and oral cavity; Peptococcus and peptic streptococcus exist in the intestine, oral cavity, Vagina and skin; Propionibacterium often exists in the skin, upper respiratory tract and vagina; Veillonella is found in the mouth, upper respiratory tract, vagina and intestines. According to the hospital's data, the detection rate of anaerobic bacteria in abdominal infections is 62%, and it accounts for 77% of appendix abscesses and post-appendectomy incision suppuration.
Anaerobic bacteria can not only cause severe chest and abdomen infections and abscesses, but also many serious soft tissue necrotic infections are almost all related to anaerobic bacteria. Therefore, the isolation and identification of anaerobic bacteria is very important in clinical diagnosis and scientific research.
Common cultivation methods
Anaerobic Chamber (Anaerobic Chamber) manufactured by LABOAO is currently recognized as a reasonable instrument for culturing anaerobic bacteria in clinical and scientific research laboratories. More and more laboratories have realized that improving efficiency and saving costs must be transferred from anaerobic tanks or anaerobic bags to anaerobic incubators. It is not like a disposable anaerobic bag or anaerobic tank, which will inevitably expose the sample to the air; while the bare-hand sleeve design of the anaerobic incubator allows free handling of samples and a strict oxygen-free environment, prompting faster samples Turnover, and a series of basic operation procedures from disassembly of materials to inoculation, cultivation, and inspection are all completed in an anaerobic environment. It provides the operator with an anaerobic environment that can be operated in the box and cultivated separately.
The anaerobic incubator is made of continuously welded stainless steel and plexiglass sealed to ensure the robustness and durability of the cabinet. The plexiglass can observe the working area in the box at a glance, and the field of vision has no blind spots. There are two oval sealed doors and sleeves on the plexiglass, which can be operated in the box through the rubber sleeves.
Operating procedures:
1. Open the door of the sample transfer room first, and put the required sample into the transfer room; when the transfer room is closed, the air will also enter the transfer room. The transfer chamber is turned into an anaerobic state through the automatic circulation method of vacuuming and adding gas (the vacuum pressure gauge can display and set the value according to the demand).
2. The rubber sleeve is also changed into an anaerobic environment through the automatic circulation method of vacuuming and adding gas (the vacuum pressure gauge can display and set the value according to the demand).
3. Open the sealed door through the sleeve, reach into the operation room to open the door of the sample transfer room, take out the sample and put it in the operation room for operation.
4. There is a power socket inside the console, which is convenient for the operator to inoculate his colonies with an inoculation loop.
5. Put the required culture dish or Erlenmeyer flask into the culture room hanging on the back of the operation room for individual culture; precise temperature control is conducive to the growth and reproduction of colonies, and finally the growth of anaerobic bacteria can be judged by naked eye or microscope Happening.
The principle of keeping the anaerobic state in the box is to fill the mixed gas into the box, and the hydrogen in the mixed gas and the remaining oxygen in the box will produce water under the action of the palladium catalyst. The cabinet has features: a microcomputer control system that displays the operating status of the entire cabinet through indicator lights; the sample transfer chamber and rubber sleeve are separately equipped with vacuum pressure gauges and oxygen exhaust indicator lights; independent over-temperature protection system; operating room Automatically press the button once to automatically exhaust oxygen to reach an anaerobic environment; HEPA filter purification and filtration; built-in gas pressure regulator and other functions. Both are used to improve the accuracy and reliability of experimental results. Ensure that the anaerobic incubator provides users with a strict anaerobic environment. Provide strong technical support for its follow-up clinical or scientific research experiments!